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anti-human clusterin rabbit polyclonal antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti-human clusterin rabbit polyclonal antibody
    Anti Human Clusterin Rabbit Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+human+clusterin+antibody/anti+clu/pm21396851-50-34-39
    Average 90 stars, based on 1 article reviews
    anti-human clusterin rabbit polyclonal antibody - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Immunodetection:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).

    TUNEL Assay:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).

    Selection:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).

    Cytometry:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).

    Immunocytochemistry:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).

    Labeling:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).

    Western Blot:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).

    Molecular Weight:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).

    Control:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).

    Expressing:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).

    Staining:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).

    Immunolabeling:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).

    Microscopy:

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.
    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.. The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).The specificity of this antibody was tested using SDS gel electrophoresis, and western blotting of total sperm proteins performed as previously described (Muciaccia et al., 2005).



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    Figure 1 <t>Clusterin</t> immunodetection and TUNEL assay before (total) and after (selected) motile spermatozoa swim-up selection, from the same healthy normotensive subject. Swim-up selection drastically reduces the amount of high-clusterin-positive cells as indicated by the very low number of events revealed in the M3 region by flow cytometry (A and B). By immunocytochemistry, normal spermatozoa labeled in the acrosomal region only, possibly corresponding to the flow cytometry M2 region (large dotted square), were observed in the total sperm population, together with morpho- logical abnormal spermatozoa with highly clusterin labeled head and flagellum, possibly corresponding to the M3 region of flow cytometry (small dotted square) (C). Highly labeled cells were not present after swim-up selection of motile spermatozoa (D). Swim-up selection drastically reduces also the amount of TUNEL-positive spermatozoa (E and F) as indicated by the low number of events revealed in the M4 region by flow cytometry. In the upper central insert, a western blot confirming the molecular weight of sperm clusterin bands specifically recognized by the primary antibody used, is visible. Bands at 80 kDa represent the heterodimeric form of the protein, while the single chains migrate at 40 kDa.
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    Image Search Results


    Figure 1 Clusterin immunodetection and TUNEL assay before (total) and after (selected) motile spermatozoa swim-up selection, from the same healthy normotensive subject. Swim-up selection drastically reduces the amount of high-clusterin-positive cells as indicated by the very low number of events revealed in the M3 region by flow cytometry (A and B). By immunocytochemistry, normal spermatozoa labeled in the acrosomal region only, possibly corresponding to the flow cytometry M2 region (large dotted square), were observed in the total sperm population, together with morpho- logical abnormal spermatozoa with highly clusterin labeled head and flagellum, possibly corresponding to the M3 region of flow cytometry (small dotted square) (C). Highly labeled cells were not present after swim-up selection of motile spermatozoa (D). Swim-up selection drastically reduces also the amount of TUNEL-positive spermatozoa (E and F) as indicated by the low number of events revealed in the M4 region by flow cytometry. In the upper central insert, a western blot confirming the molecular weight of sperm clusterin bands specifically recognized by the primary antibody used, is visible. Bands at 80 kDa represent the heterodimeric form of the protein, while the single chains migrate at 40 kDa.

    Journal: Human reproduction (Oxford, England)

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.

    doi: 10.1093/humrep/des173

    Figure Lengend Snippet: Figure 1 Clusterin immunodetection and TUNEL assay before (total) and after (selected) motile spermatozoa swim-up selection, from the same healthy normotensive subject. Swim-up selection drastically reduces the amount of high-clusterin-positive cells as indicated by the very low number of events revealed in the M3 region by flow cytometry (A and B). By immunocytochemistry, normal spermatozoa labeled in the acrosomal region only, possibly corresponding to the flow cytometry M2 region (large dotted square), were observed in the total sperm population, together with morpho- logical abnormal spermatozoa with highly clusterin labeled head and flagellum, possibly corresponding to the M3 region of flow cytometry (small dotted square) (C). Highly labeled cells were not present after swim-up selection of motile spermatozoa (D). Swim-up selection drastically reduces also the amount of TUNEL-positive spermatozoa (E and F) as indicated by the low number of events revealed in the M4 region by flow cytometry. In the upper central insert, a western blot confirming the molecular weight of sperm clusterin bands specifically recognized by the primary antibody used, is visible. Bands at 80 kDa represent the heterodimeric form of the protein, while the single chains migrate at 40 kDa.

    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.

    Techniques: Immunodetection, TUNEL Assay, Selection, Cytometry, Immunocytochemistry, Labeling, Western Blot, Molecular Weight

    Figure 2 Flow cytometric analysis of sperm clusterin positivity and DNA fragmentation in control and hypertensive subjects (healthy normotensive subjects, n ¼ 25; hypertensive subjects, n ¼ 25). In A and B representative FACS profiles from control and hypertensive subjects are, respectively, shown, while the numbers indicate the mean value of each group studied. (A) Histograms of clusterin expression profile (x-axis: fluorescence intensity; y-axis: number of events). Cells located within the M1 region are clusterin negative. Cells located within the M2 and M3 regions are intermediate (M2) and highly (M3) clusterin positive. (B) Dot plots of spermatozoa double labeled for clusterin and TUNEL assay (x-axis: intensity of TUNEL fluores- cence; y-axis: intensity of clusterin fluorescence). Graphs were divided into four quarters to define the positivity for different staining. LL (low left): spermatozoa with null or medium fluorescence intensity for clusterin and TUNEL negative. UR (upper right): spermatozoa highly positive for both clusterin and TUNEL. UL (upper left): spermatozoa highly positive for clusterin and TUNEL negative; LR (low right): spermatozoa with null or medium fluorescence intensity for clusterin and TUNEL positive. (C) In the histogram, differences for clusterin (M2 and M3), TUNEL and TUNEL/clusterin M3 mean values (% gated) between the control and the hypertensive groups are shown. **P , 0.01.

    Journal: Human reproduction (Oxford, England)

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.

    doi: 10.1093/humrep/des173

    Figure Lengend Snippet: Figure 2 Flow cytometric analysis of sperm clusterin positivity and DNA fragmentation in control and hypertensive subjects (healthy normotensive subjects, n ¼ 25; hypertensive subjects, n ¼ 25). In A and B representative FACS profiles from control and hypertensive subjects are, respectively, shown, while the numbers indicate the mean value of each group studied. (A) Histograms of clusterin expression profile (x-axis: fluorescence intensity; y-axis: number of events). Cells located within the M1 region are clusterin negative. Cells located within the M2 and M3 regions are intermediate (M2) and highly (M3) clusterin positive. (B) Dot plots of spermatozoa double labeled for clusterin and TUNEL assay (x-axis: intensity of TUNEL fluores- cence; y-axis: intensity of clusterin fluorescence). Graphs were divided into four quarters to define the positivity for different staining. LL (low left): spermatozoa with null or medium fluorescence intensity for clusterin and TUNEL negative. UR (upper right): spermatozoa highly positive for both clusterin and TUNEL. UL (upper left): spermatozoa highly positive for clusterin and TUNEL negative; LR (low right): spermatozoa with null or medium fluorescence intensity for clusterin and TUNEL positive. (C) In the histogram, differences for clusterin (M2 and M3), TUNEL and TUNEL/clusterin M3 mean values (% gated) between the control and the hypertensive groups are shown. **P , 0.01.

    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.

    Techniques: Control, Expressing, Labeling, TUNEL Assay, Staining

    Figure 3 Confocal micrographs of ejaculated human spermatozoa labeled for clusterin (red fluorescence) and for DNA fragmentation (green fluor- escence). Blue fluorescence identifies nuclei (bar ¼ 10 mm). Phase contrast (A) and double immunolabeling for clusterin and TUNEL (B) of the same microscopic field of total sperm population from a hypertensive subject. The arrow indicates two clusterin-positive spermatozoa and the arrowhead points to a clusterin- and TUNEL-negative spermatozoon. Several cells are positive for both clusterin and TUNEL. (C–E): Higher magnification of a total sperm population from a hypertensive subject; C: merged image of the phase contrast with the three fluorescence channels; D: single fluorescent channel for clusterin; E: single fluorescent channel for damaged DNA. The arrows indicate a spermatozoon positive for both clusterin and TUNEL. The arrowheads indicate spermatozoa positive only for clusterin. The frames highlight spermatozoa that are only TUNEL positive. F–H details of a sperm- atozoon showing strong morphological alterations and DNA damage; (F) merged image of the phase contrast microscopy with the three fluorescent channels; (G) single fluorescent channel for clusterin; (H) single fluorescent channel for fragmented DNA.

    Journal: Human reproduction (Oxford, England)

    Article Title: Higher clusterin immunolabeling and sperm DNA damage levels in hypertensive men compared with controls.

    doi: 10.1093/humrep/des173

    Figure Lengend Snippet: Figure 3 Confocal micrographs of ejaculated human spermatozoa labeled for clusterin (red fluorescence) and for DNA fragmentation (green fluor- escence). Blue fluorescence identifies nuclei (bar ¼ 10 mm). Phase contrast (A) and double immunolabeling for clusterin and TUNEL (B) of the same microscopic field of total sperm population from a hypertensive subject. The arrow indicates two clusterin-positive spermatozoa and the arrowhead points to a clusterin- and TUNEL-negative spermatozoon. Several cells are positive for both clusterin and TUNEL. (C–E): Higher magnification of a total sperm population from a hypertensive subject; C: merged image of the phase contrast with the three fluorescence channels; D: single fluorescent channel for clusterin; E: single fluorescent channel for damaged DNA. The arrows indicate a spermatozoon positive for both clusterin and TUNEL. The arrowheads indicate spermatozoa positive only for clusterin. The frames highlight spermatozoa that are only TUNEL positive. F–H details of a sperm- atozoon showing strong morphological alterations and DNA damage; (F) merged image of the phase contrast microscopy with the three fluorescent channels; (G) single fluorescent channel for clusterin; (H) single fluorescent channel for fragmented DNA.

    Article Snippet: Rabbit polyclonal anti-human clusterin antibody (H-330, #sc-8354; Santa Cruz Biotechnology, Germany), reacting with clusterin a and b chains, was used.

    Techniques: Labeling, Immunolabeling, TUNEL Assay, Microscopy